During the last 10 years, we have observed an exponential growth of DNA sequencing methods with the development of Next Generation Sequencing (NGS). Currently in use are mostly second and third generation NGS methods, better known as high throughput sequencing or massive sequencing.
NGS opens nowadays a wide landscape of possibilities in several fields related with biological science and public health.
Massive sequencing is allowing incredible advances in several disciplines such as:
- Clinical microbiology and epidemiology
- Human microbiome communities and their relationships with health and disease
- Microbiological safety in Public Health in agro-food industry
- Plague control
- Human genetics
The FISABIO Sequencing and Bioinformatics Service currently operates two Illumina MiSeq sequencing machines and one Illumina NexSeq500. Moreover the Service is part of the Oxford Nanopore MinIon access program, one of the last third generation sequencers.
The service also has access to molecular biology laboratories and all accessories for biological sample processing, nucleic acids extraction and sequencing libraries preparation.
The work flow goes through four separated areas to avoid cross contamination before and after amplification steps.
The FISABIO Sequencing and Bioinformatics Service is equipped with informatics facilities for data managing that are well adapted to the needs of massive sequencing data. Currently, the Service is equipped with a 32 Cores/64Gb Ram, and a 96 Cores/512Gb Ram servers respectively, backup units, several desktop computers and has personnel in charge of sequencing quality assessment and data delivery.